LOCUS       Exported                3937 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0041_regulator_luxR_corrected
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Daniel Gerngross
  TITLE     piG0041
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      12..98
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    280..471
                     /note="ROP"
     misc_feature    573..713
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(884..1503)
                     /direction=LEFT
                     /note="pBR322_origin"
     oriT            1523..1631
                     /note="incP origin of transfer"
     CDS             complement(1821..2681)
                     /note="AmpR"
     promoter        complement(2682..2773)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2846..2940
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        2984..3018
                     /note="const. promoter (J23100)"
     RBS             3027..3038
                     /note="RBS (B0034)"
     CDS             3045..3800
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN*"
     terminator      3809..3937
                     /note="directional double term (B0015)"
ORIGIN
        1 attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggc
      121 gcgcccccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
      181 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
      241 ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc
      301 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
      361 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc tgcacgacca cgctgatgag
      421 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
      481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
      541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
      601 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      661 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
      721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1501 tcttttctac ggggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg
     1561 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     1621 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     1681 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     1741 cgaagcaggg ttatgcagcg gaaaaggaca agggtcgtcc aaaaaaaaag gctccaaaag
     1801 gagcctttaa ttgtatcggt ttaccaatgt ttaatcaggc tcgcgccaat ttccgcaatc
     1861 tgacggttac gttcatccat cgtcgcctgg ctgccggtgg tataaatcac cacaatacgg
     1921 ctcggtttac catccgggcc cagcgccgca ataatgccac ggctgccacg ttcgcccgca
     1981 ccgcttttat ccgcaataaa ccagccagcc ggcagcgcgc tacgcagcag cggacccgcc
     2041 actttatccg cttccatcca atcaatcagt tgctggcggc ttgccagggt cagcagctca
     2101 cccgtcagca gtttacgcag ggtggtcgcc attgctgccg gcatggtggt atcacgttca
     2161 tcgttcggaa tcgcttcgtt cagttccggt tcccaacgat ccagacgggt cacatgatcg
     2221 cccatgttat gcagaaacgc ggtcagttct ttcggaccgc caatggtcgt cagcagcagg
     2281 ttcgccgcgg tgttatcgct catggtaatc gccgcgctgc acagttcacg cacggtcatg
     2341 ccatcggtca gatgtttttc ggtcaccggg ctatattcca ccagatcgtt ctggctataa
     2401 tgaatacgac ggcccagttg ttcctggccc gcatccacac ggctcagaac cgcaccgcac
     2461 agcagcactt taaaggtgct catcatcgga aaacgttctt ccggacgaaa agattccaga
     2521 attttgccgc tgttcagatc cagttcaata tagcccacgc gcgcacccag ttgatcttcc
     2581 gcatctttca ctttcaccag ggtttccgga tgcgcaaaca ccggcaggca aaacgccgca
     2641 aaaaacggaa tcagcgccac acgaaaatgc tgaatgctca tactcttcct ttttcaatat
     2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2761 aaaaataaac aaatagggga attaaaaaaa agcccgctca ttaggcgggc tactaattta
     2821 aatcgtaatt attggggacc cctggattct caccaataaa aaacgcccgg cggcaaccga
     2881 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2941 caagactagt cgccagggtt ttcccagtca cgacgcggcc gcattgacgg ctagctcagt
     3001 cctaggtaca gtgctagcta ctagagaaag aggagaaata ctagatgaaa aacataaatg
     3061 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta
     3121 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca
     3181 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat
     3241 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta
     3301 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat
     3361 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta
     3421 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact
     3481 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag
     3541 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa
     3601 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa
     3661 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca
     3721 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc
     3781 catactttaa aaattaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3901 actggctcac cttcgggtgg gcctttctgc gtttata
//
